In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAMlabeled adaptors using the NxSeq® DNA Sample Prep kit, comparing the manual method with an automated library prep protocol on the PIPETMAX. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has been used to create useful animal models for studying complex diseases, as well as correcting or inducing disease states within a cell. This technical note describes how PLATEMASTER and EXTRACTMAN can be used in the rapid enrichment and clonal selection of CRISPR modified cells.
This application note presents a new approach for the cleanup of DNA fragments from small volumes of liquid combining the innovative design of EXTRACTMAN® with the convenience of a DNA purification using MACHEREY-NAGEL’s proven NucleoMag® technology.
RNA-binding proteins (RBPs) are involved in many key cellular processes, but identification of the native binding partners (target RNAs) can be challenging. In this application note, we show that EXTRACTMAN can be used to efficiently and specifically enrich endogenously expressed ribonucleoprotein complexes.
EXTRACTMAN® improved isolation speed nine-fold and produced equivalent or better yields of purified DNA compared to tube-based isolation methods. In this application note, four DNA samples from two different organisms (mammalian and plant) were processed in parallel with no cross-contamination.
This application note describes how PIPETMAX® with qPCR Assistant is a highly proficient pipetting workstation that reduces the potential for cross-contamination and other human induced errors.
To make the qPCR technique more reproducible, scientists need liquid handling protocols that can be readily automated, modified, and transferred between different instruments without affecting the quality of the data itself.
Pipetting insights and digital records of your protocols are at your fingertips with TRACKMAN® Connected, a tablet with accessories and apps that makes pipetting on microplates faster, with greater traceability and reproducibility.
In this case study, learn how one molecular biology and genomics lab is saving time, improving data traceability, and standardizing protocols by using the TRACKMAN Connected pipetting system.