In this technical note, the use of a PIPETMAX® automated liquid handling system is described for the pipetting of the plasma from the whole blood sample. PIPETMAX can be used to aspirate a precise volume of the plasma and transfer this to a suitable analysis or storage tube. If the specific laboratory workflow will benefit from it, then diluent can also be added to the analysis tubes, and the plasma can be mixed into the diluent as part of the same method.
The Coomassie (Bradford) assay is a well-established method for protein quantification that utilizes a commonly placed UV/Vis spectrophotometer. Streamlining the manual pipetting steps through
automated liquid handling results in less data variability and improvements in workflow efficiencies.
Gilson and Funai show a preview of the soon-to-be-released Gilson Digital Dispenser. This is a non-contact dispenser capable of dispensing DMSO and aqueous solutions from pL to µL. The large dynamic range makes it ideal for dose-response studies, serial dilutions, and assay miniaturization. In this webinar, our attending experts demonstrate the functionality of the new Digital Dispenser with a cell-based assay example.
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease caused by the death of motor neurons. A hallmark of ALS is low expression of neurofilament light chain (NFL) in motor neurons. A high-throughput phenotypic assay using patient-derived induced pluripotent stem cells (iPSCs) was used to screen over 6,000 compounds. This poster demonstrates how automation of serial dilutions and dosing on PIPETMAX provides a more accurate and less labor-intensive alternative to manual pipetting for rescreening.
In environments requiring higher throughput levels, manual pipetting methods used in sample preparation, transfer, and mixing can sometimes be time-consuming and prone to procedural errors. Consider these automated liquid handling instruments if you need to increase your sampling throughput without compromising accuracy or repeatability.
Increased demand and the need for consistent results require every lab to deploy automated liquid handling in their workflows. Here’s what to consider.
Kinase profiling is a necessary process to confirm inhibitor selectivity and assess off-target activity. The arduous task of optimizing kinase reactions and maintaining a library of enzymes often results in the outsourcing of kinase profiling activities. This poster breaks down a flexible workflow for the automation of bioluminescent kinase selectivity profiling.
Automation of the Kinase Selectivity Profiling System with PIPETMAX, in conjunction with automated detection and data analysis methods, offers a streamlined workflow for single-point and dose-response evaluation of potential cancer therapies. Learn more in this application note.
The Bradford Assay is a common universal protein concentration determination technique. In this app note, you'll learn how the automation of this qualitative application shows comparability between manual and automated results for preparing a standard curve and samples for quantification of protein in solution.