Gilson Connect
Liquid chromatography (LC) is the standard method used for purifying products in the discovery stages, as it is cost-effective, relatively simple to perform, and reliable. However, obtaining high purity and yield using this approach often comes at the cost of throughput, as purification runs must be lengthened to achieve greater separation between components. This article explores how users can enhance fraction collection efficiency and streamline the purification process by using the latest advances.
Using the right systems can improve sample integrity in your sample separation workflows, helping labs to have greater confidence in their results. Here we outline what to look for in your sample separation instruments to remove risks of common threats to sample integrity.
In this video, you will learn about the benefits of Centrifugal Partition Chromatography (CPC) as it relates to other forms of traditional sample separation. Without solid support, liquid-liquid chromatography allows for repeat injections of samples with 100% recovery of the materials--and you'll never need to replace any silica columns. CPC columns tackle tough separations of complex samples in a single step without prior purification, making it an ideal choice for natural product isolation, protein purification, and more.
While more than 220 viruses are known to infect humans, clinically approved antiviral drugs are available for only a dozen of these viruses. CPC is an ideal solution for natural compound investigations. Gilson, in collaboration with Brazilian researchers, recently performed preliminary experiments demonstrating the antiviral activity of compounds that were fractionated from a plant crude extract using CPC. Read more about the study here.
In this article, we hear from an industry leader on their experience using centrifugal partition chromatography (CPC) in the pharmaceutical industry, and how it's a method well-suited for producing highly active molecules that meet safety and performance requirements.
Lignin is an abundant phenylpropanoid polymer found in all plant matter. It has historically been treated as an impurity in the paper pulping and ethanol biorefinery industries where it is burned as a low-value fuel or sold at low prices. However, developing higher-value applications of lignin would help biorefineries operate at higher efficiencies. In this app note, a two-dimensional separation is conducted using centrifugal partition chromatography (CPC), which helps demonstrate the value of CPC as a tool for gaining deeper insights into depolymerization mixtures for biomass facilities.
Forsythiaside A, a phenylethanoid glycoside, is one of the major active constituents extracted from the air-dried fruits of Forsythia suspensa and has been reported to possess a wide range of pharmacological properties, including anti-inflammatory and antioxidant activities. This application note discusses a simple, fast, and cost-efficient method to prepare pure compounds using a centrifugal partition chromatography (CPC) system.
Whether you’re purifying at small-scale preclinical research levels or larger manufacturing levels, Gilson’s scalable lineup of high performance liquid chromatography (HPLC) and centrifugal partition chromatography (CPC) systems has you covered. These solutions are dependable, flexible, and precise. Our team of experts is here to help you find the one that is right for you.
The rise of biorenewable alternatives and petroleum/fossil-derived chemicals have led to a growing interest in lignin valorization. In this webinar, Gilson's Dr. Manar Alherech demonstrates the feasibility of isolating valuable compounds from an oxidative lignin depolymerization matrix using centrifugal partition chromatography (CPC).
Artemisinin is a sesquiterpene lactone obtained from sweet wormwood, Artemisia Annua L. The extract of the leaves of Artemisia Annua L. is used in ancient Chinese herbal therapy for malarial fevers. Artemisinin has been identified as an active antimalarial compound. In this application note, the use of CPC coupled with a preparative liquid chromatography system equipped with UV-DAD and mass spectrometry (MS) detectors, illustrates the success of such technologies to isolate active compounds from complex mixtures.
