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Preparative high performance liquid chromatography (HPLC) is a well-established method for purifying target compounds. Method development is usually done at a smaller scale to define sufficient resolution between the peaks without wasting large amounts of sample. It is common to scale-up the purification from a smaller column to a larger preparative one - learn more in this technical note.
Liquid chromatography (LC) is the standard method used for purifying products in the discovery stages, as it is cost-effective, relatively simple to perform, and reliable. However, obtaining high purity and yield using this approach often comes at the cost of throughput, as purification runs must be lengthened to achieve greater separation between components. This article explores how users can enhance fraction collection efficiency and streamline the purification process by using the latest advances.
The Institute for Research and Innovation in Biomedicine (IRIB) focuses on developing innovative technologies for diagnosing, treating, and preventing diseases. In this case study, we explore how the researchers at IRIB are using preparative HPLC in their peptide purification drug discovery applications.
Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. In this tech note, we demonstrate the use of a VERITY 271 LCMS system to perform mass-based purification of target peptides from a mixture. The system is controlled using TRILUTION® LC Software, which provides real-time and post-run MS and UV data.
Using the right systems can improve sample integrity in your sample separation workflows, helping labs to have greater confidence in their results. Here we outline what to look for in your sample separation instruments to remove risks of common threats to sample integrity.
Custom manufactured oligonucleotides require additional purification steps after synthesis to separate the full-length product from failing sequences and small impurities. In this infographic, we highlight how to accelerate purification for enhanced productivity and scalability with automation.
Oligonucleotides, or oligos, are at the core of some of the most life-changing molecular biology tools used today. From qPCR to antisense gene therapy, oligos have changed what scientists can achieve through synthetic and molecular biology. While synthesis methods continue to advance, one significant challenge remains: oligo purification. Here, we explore the most common approaches to oligo purification, and explain how the right HPLC system can overcome any current and future challenges and help you achieve more accurate results.
Forsythiaside A, a phenylethanoid glycoside, is one of the major active constituents extracted from the air-dried fruits of Forsythia suspensa and has been reported to possess a wide range of pharmacological properties, including anti-inflammatory and antioxidant activities. This application note discusses a simple, fast, and cost-efficient method to prepare pure compounds using a centrifugal partition chromatography (CPC) system.
Whether you’re purifying at small-scale preclinical research levels or larger manufacturing levels, Gilson’s scalable lineup of high performance liquid chromatography (HPLC) and centrifugal partition chromatography (CPC) systems has you covered. These solutions are dependable, flexible, and precise. Our team of experts is here to help you find the one that is right for you.
Researchers are rushing to identify, target, and analyze peptide functions for drug development. Here is what you should know about peptide purification.
